Part:BBa_K3956010:Design

The Scl2 gene itself is made of several parts: the V domain, the triple helical CL domain, and the tail. When building the plasmid to express Scl2, we had to add a 5’UTR in front of the codon to make sure the triple helical structure was produced.

Source: Xu, Y., Keene, D. R., Bujnicki, J. M., Höök, M., & Lukomski, S. (2002). Streptococcal Scl1 and Scl2 proteins form collagen-like triple helices. Journal of Biological Chemistry, 277(30), 27312-27318.